Evaluation of the fungistatic activity of biopreparations against the main pathogens of highbush blueberry (Vaccinium corymbosum L.)
DOI:
https://doi.org/10.36495/2312-0614.2026.3.29-36Keywords:
biofungicides, fungistatic effect, pathogens, biological control, Fusarium, Neopestalotiopsis, RhizoctoniaAbstract
Goal. To evaluate the fungistatic activity of the preparations LS-1, WS (silver lactate, 1 g/l silver) and Mikosan, LC (alkaline extract of the aphyllophorous fungus Fomes fomentarius, 30 g/l + glucans, 6 g/l) against fungal pathogens of the genera Neopestalotiopsis, Fusarium and Rhizoctonia under in vitro conditions. To analyse the dynamics of radial mycelial growth and to determine the dose-dependent effect. Methods. The studies were conducted under laboratory conditions. The ‘poisoned medium’ method was used to assess the fungistatic activity of the preparations LS-1, WS and Mikosan, LC. Pathogen isolates belonging to the genera Neopestalotiopsis, Fusarium and Rhizoctonia, isolated from blueberry plant material, were studied. Results and discussion. It was found that LC-1, WS (at concentrations of 0.25% and 0.5%) had low fungistatic activity, which was limited to the initial stages of cultivation (3—6 days). By day 10, the mycelium of all pathogens had fully recovered from the inhibition. The biofungicide Mikosan, LC demonstrated a clearly dose-dependent effect. At a concentration of 0.5%, a moderate fungistatic effect was observed against all the pathogens studied, whilst at a concentration of 5%, it ensured complete inhibition of mycelial growth throughout the entire observation period (10 days), demonstrating high fungicidal activity. The highest sensitivity to Mikosan, LC was observed in R. solani. The results obtained confirm a difference in fungistatic activity between the preparations: LS-1, WS has a weak effect, whilst Mikosan, LC demonstrates a pronounced dose-dependent fungistatic and fungicidal effect against various pathogens. Conclusions. It has been established that Mikosan, LC exhibits a pronounced dose-dependent effect. At a concentration of 0.5%, it exerts a fungistatic effect, accompanied by a significant reduction in the rate of radial growth and a prolongation of the lag phase of development by 2—3 days. At a concentration of 5%, a fungicidal effect was observed against all the pathogens studied. The results obtained provide scientific justification for the use of Mikosan, LC as a promising agent for the biological control of fungal pathogens.
References
1. Yareshchenko O., Pukshyn O., Grynyk I., Tereshchenko Y. (2025) Blueberry growing in Ukraine — development and trends. Acta Horticulturae, 1440, 407–412. DOI: https://doi.org/10.17660/ActaHortic.2025.1440.56
2. Kalt W., Cassidy A., Howard L.R., Krikorian R., April J Stull, Tremblay F., Zamora-Ros R. (2020). Recent research on the health benefits of blueberries and their anthocyanins. Advances in Nutrition, 11(2), 224–236. https://doi.org/10.1093/advances/nmz065
3. Haralson J.C., Brannen P.M., Scherm H. (2021). Survey of potential root pathogens in softwood cuttings collected from Georgia blueberry nurseries. Plant Health Progress, 23(1), 87–92. https://doi.org/10.1094/PHP–06–21–0094-S
4. Sneh B., Burpee L., Ogoshi A. (1991). Identification of Rhizoctonia Species. St. Paul: APS Press. 133 p.
5. Brent K.J., Hollomon D.W. (2007). Fungicide resistance in crop pathogens: how can it be managed? Brussels: FRAC. 60 p.
6. Lucas J.A., Hawkins N.J., Fraaije B.A. (2015). The evolution of fungicide resistance. In: Advances in Applied Microbiology, 90, Elsevier, 29-92. https://doi.org/10.1016/bs.aambs.2014.09.001
7. Prodorutti D., Pertot I., Giongo L., Gessler C. (2007). Highbush blueberry: Cultivation, protection, breeding and biotechnology. The European journal of plant science and biotechnology, 1(1), 44-56.
8. Percival D.C., Dawson J.K. (2008, July). Foliar disease impact and possible control strategies in wild blueberry production. Acta Hortic, 810, 345-354. https://doi.org/10.17660/ActaHortic.2009.810.45
9. EFSA (European Food Safety Authority), Carrasco Cabrera L., Di Piazza G., Dujardin B., Marchese E., Medina Pastor P. (2024). The 2022 European Union report on pesticide residues in food. EFSA Journal, 22(4), e8753. https://doi.org/10.2903/j.efsa.2024.8753
10. Abbey J., Percival D., Jaakola L., Asiedu S.K. (2024). Efficacy, persistence and residue levels of fungicides for Botrytis control in wild blueberry. Crop Protection, 179, 106633. https://doi.org/10.1016/j.cropro.2024.106633
11. Migura V.V., Masyuk O.M. (2024). The influence of varietal characteristics of blueberry on the development of fungal diseases in the steppe zone of Ukraine. Issues of steppe forestry and forest reclamation of soils, 25, 98-110. https://doi.org/10.15421/442510
12. Cline W.O. (2014). New and emerging diseases of blueberry. Acta Horticulturae, 1017, 45-49 https://doi.org/10.17660/ActaHortic.2014.1017.2
13. Gosch C. (2006). Mummy berry disease (Monilinia vaccinii-corymbosi) on highbush blueberries in Europe. Acta Horticulturae, 715, 469-472 https://doi.org/10.17660/ActaHortic.2006.715.71
14. Schilder A.M.C., Hanson E.J., Hancock J.F. (2006). An integrated approach to disease control in blueberries in Michigan. Acta Horticulturae, 715, 481-488. https://doi.org/10.17660/ActaHortic.2006.715.73
15. Cook R.J., Baker K.F. (1983). The Nature and Practice of Biological Control of Plant Pathogens. St. Paul: APS Press. 539 p.
16. Harman G.E., Howell C.R., Viterbo A., Chet I., Lorito M. (2004). Trichoderma species — opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2, 43-56. https://doi.org/10.1038/nrmicro797
17. Vinale F., Sivasithamparam K., Ghisalberti E.L., Marra R., Woo Sh.L., Lorito M. (2008). Trichoderma-plant-pathogen interactions. Soil Biology & Biochemistry, 40, 1-10. https://doi.org/10.1016/j.soilbio.2007.07.002
18. Lisova G., Golosna L., Afanasieva O., Lutsko A. (2015). Khvoroby lokhyny vysokorosloi (Vaccinium corymbosum L.) [Disease of blueberry (Vaccinium corymbosum L.)]. Quarantine and Plant Protection, (1), 11-14. https://kr.ipp.gov.ua/index.php/journal/issue/view/42 (in Ukrainian).
19. Li J.Y., Harper J.K., Grant D.M., Tombe B.O., Bashyal B., Hess W.M., Strobel G.A. (2001). Ambuic acid, a highly functionalized cyclohexenone with antifungal activity from Pestalotiopsis spp. and Monochaetia sp. Phytochemistry, 56, (5), 463-468. https://doi.org/10.1016/S0031-9422(00)00408-8
20. Maharachchikumbura S.S.N., Hyde K.D., Groenewald J.Z., Xu J., Crous P.W. (2014). Pestalotiopsis revisited. Studies in Mycology, 79, 121-186. https://doi.org/10.1016/j.simyco.2014.09.005
21. Liu Y.H., Lin T., Ye C.S., Zhang C.Q. (2014). First Report of Fusarium Wilt in Blueberry (Vaccinium corymbosum) Caused by Fusarium oxysporum. Plant Disease, 98(8), 1158. https://doi.org/10.1094/PDIS-02-14-0167-PDN
22. Wright E.R., Rivera M.C., Campanella E.R., Farinon O.M., Berretta M.F., Perez B.A. (2014). Fusarium branch blight on highbush blueberry in Argentina. African Journal of Biotechnology, 13(51), 4628-4634. https://doi.org/10.5897/AJB2014.14156
23. Haralson J.C. (2013). Chemical control of Cylindrocladium and Rhizoctonia root rots in blueberry propagation. Crop Protection, 44, 1-5. https://doi.org/10.1016/j.cropro.2012.09.017
24. Haralson J.C., Brannen P.M., Sanders W., Scherm H. (2023). Changes in production practices used for disease management in blueberry nurseries in Georgia, USA, over a 15 year period. HortTechnology, 33(3), 268-277. https://doi.org/10.21273/HORTTECH05154-22
25. Hahn M. (2014). The rising threat of fungicide resistance in plant pathogenic fungi: Botrytis as a case study. J Chem Biol., 7, 133-141 https://doi.org/10.1007/s12154-014-0113-1
26. Shevchuk O., Vlasova O., Shevchuk I., Stefkivska Y. (2023). Vyznachennia pokaznykiv toksychnosti pestytsydiv metodom probit-analizu. [Determination of pesticide toxicity parameters using probit-analysis]. Quarantine and Plant Protection, (2), 14-19. https://doi.org/10.36495/2312-0614.2023.2.14-19 (in Ukrainian).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2026 D.S. Zlenko, O.H. Afanasieva
This work is licensed under a Creative Commons Attribution 4.0 International License.
