Species composition of detected nematodes of the genus Bursaphelenchus in Ukrainian Polissia
DOI:
https://doi.org/10.36495/2312-0614.2026.2.33-40Keywords:
nematodes, Bursaphelenchus, phytoparasitic, pine woodAbstract
Goal. To study the fauna of nematodes of the genus Bursaphelenchus in Zhytomyr and Volyn Polissia.
Methods. Samples of Scots pine wood were collected in 2021—2022 during a nematological survey in accordance with standard procedures. Nematodes were isolated from the wood using Berman’s funnel method. They were fixed with a TAF solution (triethanolamine, 2 cm³; formalin 40%, 7 cm³; distilled water, 91 cm³). Nematodes were identified on permanent water-glycerol mounts using the Seinhorst method (Seinhorst, 1959) with an XS-3330-LED MICROmed digital microscope equipped with a MICROmed MDC-500 5 MPix digital camera. using identification keys by Rühm W., Massey C. , Blinova S.L. and others.
Results. The species composition of nematodes of the genus Bursaphelenchus in the Volyn and Zhytomyr Polissya regions of Ukraine has been studied. Morphometric data were measured and, based on these, the species composition of the identified nematodes was established. A comparative analysis of the Bursaphelenchus fauna of the Zhytomyr and Volyn Polissya with that of the Chernihiv and Kyiv Polissya revealed their species similarity.
Conclusions. As part of this study, five species of wood-dwelling nematodes of the genus Bursaphelenchus, associated with stem pests, were identified in the Zhytomyr and Volyn Polissya regions; these included Bursaphelenchus chitwoodi Rühm (1956) and Bursaphelenchus leoni Baujard (1980), which were recorded in Ukraine for the first time. The frequency of occurrence of the species varied significantly, but the reasons for this variability require further investigation. The results obtained highlight the need to continue research into the species composition and potential pathogenic status of Bursaphelenchus in Ukraine, given the quarantine significance of Bursaphelenchus xylophilus, which has not yet been detected in the country.
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