The effect of symbiotic association of fungi and bacteria with the root system of European raspberry Rubus idaeus L.
DOI:
https://doi.org/10.36495/2312-0614.2025.4.19-25Keywords:
Trichoderma, Glomus, Agrobacterium, Rosaceae, mycorrhization level, biometric indicatorsAbstract
Goal. To study the effectiveness of symbiotic relationships in the «raspberry plant (Rubus L.) — mycorrhizal fungi (Trichoderma spp., Glomus sp.) — bacteria (Agrobacterium radiobacter)» system during inoculation under controlled laboratory conditions.
Methods. Analytical, laboratory and statistical. The study was conducted in the climate chamber of the «Institute of Agrobiology» LLC from 2023 to 2024, according to a scheme involving inoculation with mycorrhizal fungi of the genera Trichoderma and Glomus, as well as non-phytopathogenic Agrobacterium bacteria, applied to the soil both separately and in combinations (n = 11 experimental variants + control). The raspberry variety (Rubus idaeus L.) ‘Vognik’ of Ukrainian origin was used. The strains of mycorrhizal fungi (Trichoderma viride eko/103, T. harzianum eko/101, and Glomus sp. eko/104) were obtained from the «Institute of Agrobiology» LLC culture collection (the active ingredients in the preparations). The bacterial endosymbionts (A. radiobacter) were isolated by the authors, either directly or indirectly.
Results. Using inoculations of mycorrhizal fungi and bacteria (Trichoderma spp., Glomus sp. and A. radiobacter) separately or in combination significantly improves the main biometric indicators of R. idaeus L. raspberry plants. This includes the length and density of root hairs, the number of lateral roots and the density of mycorrhization. It also improves the morphological and morphometric parameters of the roots, such as bark thickness and conducting cylinder width. A positive effect of varying degrees of severity on the target indicators was noted after 60 days of the growing season in all experimental variants compared to the control.
Conclusions. Using separate or combined inoculations of mycorrhizal fungi (Trichoderma spp. and Glomus sp.) and bacteria (Agrobacterium radiobacter) stimulates morphological and anatomical changes in the roots of Rubus idaeus L., as well as promoting a high level of mycorrhizal activity. This symbiotic effect is due to the combined action of the microorganisms in the multicomponent system, as well as their mutual influence. These findings are of practical importance for enhancing the efficiency of raspberry cultivation in enclosed spaces.
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