Detection and identification of the virus Grapevine Leaf Roll-Associated Virus of the vine in the vineyards of the Odessa region
Goal. Detection of viral disease of the vine — twisting leaves in the vineyards in the Odessa region, as well as identification of the causative agents of this disease.
Methods. Examination of grape plantations for the presence of symptoms of viral diseases. To identify the virus twisting the leaves of the vine and identify its serotypes used molecular-biological method of polymerase chain reaction with reverse transcription (RT-PCR) and detection using gel electrophoresis.
Results. In the Odessa region, bushes of grape plants were found with characteristic symptoms of leaf curling, namely leaf blade curling, leaf color change from green to yellow in white-berry grape varieties and from green to red in red-berry. Identification of leaf twisting pathogens showed the presence of the 1st and 3rd serotypes of the vine twisting virus. It was established that among all the identified serotypes of the virus of twisting the leaves of the vine the most common is the 3rd serotype. Grape bushes of the Odessa black variety were also found with symptoms that are not typical for a curling virus. Using RT-PCR, a virus of the 9th serotype was identified that had not previously been seen in the vineyards of southern Ukraine.
Conclusion. As a result of a phytosanitary of grape plantations in the Odessa region, a viral grapevine disease, twisting of leaves, was revealed. Using RT-PCR with gel electrophoretic detection, it was established that the grape plants were infected with the virus of twisting the leaves of the vine of the 1st and 3rd serotypes. For the first time identified the 9th serotype of the virus twisting the leaves of grapes on grape plantations in the Odessa region.
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