Detection and identification of the virus Grapevine Leaf Roll-Associated Virus of the vine in the vineyards of the Odessa region

  • A. Konup National Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» NAAS, 27, 40 years of Victory, Odessa, Ukraine, 65496
  • V. Chistyakova National Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» NAAS, 27, 40 years of Victory, Odessa, Ukraine, 65496
  • L. Konup National Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» NAAS, 27, 40 years of Victory, Odessa, Ukraine, 65496
  • N. Nikolaeva National Scientific Centre «Institute of viticulture and winemaking named after V.E. Tairov» NAAS, 27, 40 years of Victory, Odessa, Ukraine, 65496
Keywords: grape viruses, of the grapevine leaf roll-associated virus, RT-PCR, grapes

Abstract

Goal. Detection of viral disease of the vine — twisting leaves in the vineyards in the Odessa region, as well as identification of the causative agents of this disease.

Methods. Examination of grape plantations for the presence of symptoms of viral diseases. To identify the virus twisting the leaves of the vine and identify its serotypes used molecular-biological method of polymerase chain reaction with reverse transcription (RT-PCR) and detection using gel electrophoresis.

Results. In the Odessa region, bushes of grape plants were found with characteristic symptoms of leaf curling, namely leaf blade curling, leaf color change from green to yellow in white-berry grape varieties and from green to red in red-berry. Identification of leaf twisting pathogens showed the presence of the 1st and 3rd serotypes of the vine twisting virus. It was established that among all the identified serotypes of the virus of twisting the leaves of the vine the most common is the 3rd serotype. Grape bushes of the Odessa black variety were also found with symptoms that are not typical for a curling virus. Using RT-PCR, a virus of the 9th serotype was identified that had not previously been seen in the vineyards of southern Ukraine.

Conclusion. As a result of a phytosanitary of grape plantations in the Odessa region, a viral grapevine disease, twisting of leaves, was revealed. Using RT-PCR with gel electrophoretic detection, it was established that the grape plants were infected with the virus of twisting the leaves of the vine of the 1st and 3rd serotypes. For the first time identified the 9th serotype of the virus twisting the leaves of grapes on grape plantations in the Odessa region.

References

Almeida R.P.P., Daane K., Bell V. (2013). Ecology and management of grapevine leafrolldisease. Frontiers in Microbiology. Vol. 4. P. 94. DOI: https://doi:10.3389/fmicb.2013.00094

Esteban A., Engel E. A., Escobar P. F. (2010). A diagnostic oligonucleotide microarray for simultaneous detection of grapevine viruses. Journal of Virological Methods. Vol. 163. Р. 445—451.

Molecular biomarker analysis — General definitions and requirements for microarray detection of specific nucleic acid sequences European and Mediterranean Plant Protection Organization (EPPO). 2013. ISO 16578. URL: http://www.eppo.org

Milkus B.N., Konup L.O., Zhunko I.D., Limanska N.V. (2005). Testuvannia deiakykh sortiv vynohradu na naiavnist zbudnyka bakterialnoho raku i virusiv korotkovuzlia ta skruchuvannia lystia. [Testing of some grape varieties for the presence of bacterial cancer and short-knot viruses and leaf curl]. Mikrobiolohichnyi zhurnal. T. 67. № 1. S. 41—48. [in Ukrainian].

Rowhani A. (2000). Simplified sample preparation method and one-tube RT-PCR for grapevine viruses. Proceedings of XIII International Council for the Study of Viruses and Virus-Like Diseases of the Grapevine. Adelaide. Р. 82.

White E.J., Venter M., Hiten N.F., and Burger J.T. (2008). Modified Cetyltrimethylammonium bromide method improves robustness and versatility: the benchmark for plant RNA extraction. Biotechnology Journal. Vol. 3(11). Р. 1424—1428.

Fatima Osmana, Christian Leutenegger, Deborah Golino, Adib Rowhani. (2008). Comparison of low-density arrays, RT-PCR and real-time TaqMan® RT-PCR in detection of grapevine viruses. Journal of Virological Methods. Vol. 149. Р. 292—299.

Zhunko I.D., Limanska N.V., Milkus B.N., Ivanytsia V.O. (2015). Virusy ta virusni khvoroby vynohradu (VITIS SP.). [Viruses and viral diseases of grapes (VITIS SP.)]. Mikrobiolohiia i biotekhnolohiia. № 3. S. 6—17. [in Ukrainian].

Guta I.C., Buciumeanu E.C., Gheorghe R.N., Teodorescu A. (2010). Solutions to eliminate Grape vine leafroll–associated virus serotype 1+3 from Vitis vinifera cv. Rabai Magaraci. Romanian Biotechnological Letters, Vol. 15. Р. 72—78.

Published
2019-03-20
How to Cite
Konup, A., Chistyakova, V., Konup, L., & Nikolaeva, N. (2019). Detection and identification of the virus Grapevine Leaf Roll-Associated Virus of the vine in the vineyards of the Odessa region. Quarantine and Plant Protection, (3-4), 13-16. https://doi.org/10.36495/2312-0614.2019.3-4.13-16
Section
SCIENTIFIC RESEARCH