The resistance of potato varieties to dry fusarium rot in vitro

  • S. Bomok Institute of Plant Protection of NAAS, 33, Vasylkivska str., Kyiv, Ukraine, 03022
Keywords: evaluation, stability, damage, potatoes, dry fusarium rot, Fusarium ssp, selection, variety

Abstract

Goal. Determine the resistance of potato varieties against dry Fusarium rot under conditions of artificial infection in the laboratory.

Methods. Assessment of the resistance of potato tubers against dry Fusarium rot was carried out with artificial infection by the pathogen, according to the methodology of phytopathological studies on artificial infection of plants. The studied varieties were grown in the fields of Polesye of Ukraine. The laboratory experiment was carried out in 2019 and was repeated twice in May (potato yield — 2018) and August (potato yield — 2019). Tubers of each grade (5 pcs.) Injured in three places with a metal rod to a depth of 10 mm. An inoculum of a fungus of the genus Fusarium, a concentration of 1 × 105 conidia / ml, was injected into these holes with a syringe. The infected tubers were wrapped in filter paper, moistened and placed in desiccators, which were transferred to a thermostat for incubation (at a temperature of 22—24°C and a humidity of 75—80%). Measurements of the degree of damage were performed three weeks after ino­culation. Tuber resistance was determined on a 9 point scale.

Results. In seventeen varieties of potatoes of foreign and Ukrainian selection, the degree of resistance against dry Fusarium rot ranged from 3.8% to 15.7%, compared with the control variant 51.6%. Eleven varieties were highly resistant: Barvin, Belmando, Bella Rossa, LaBelle (German selection) Glazurny, Cimmeria, Flooding, Tiras (Ukrainian selection) Carrera, Marlene (Dutch selection) Sorai (Belgian selection). Six varieties were identified as resistant: Wendy, Tuscany (German selection) Monte Carlo (Danish selection), Dymka, Slavyanka, Shchedrik (Ukrainian selection). One variety is unstable (control) Treasury (Ukrainian selection). The studied varieties in the ripeness group were early and medium early. Rotting of tubers of early varieties amounted to 3.8—15.7% and a control of 51.6%, medium early — 6.5—11.7%. So, with artificial infection, varieties of potatoes of the early ripening group have a higher percentage of dry Fusarium rot damage than varieties of the mid-early ripening group.

Conclusion. The studied varieties grown in the conditions of Polesye of Ukraine are highly resistant and resistant to dry Fusarium rot (Fusarium spr.) Potatoes and can be recommended for cultivation on farms and for seed purposes.

References

Burkhart P.V., Svavarsdottir E.K., Rayens M.K., Oakley M.G., Orlygsdottir B. (2009) Adolescents with asthma: predictors of quality of life. Journal of Advanced Nursing 65(4). P.860–866 [in English]

Nemerytska L. V., Polozhenets V. M., Derecha O. A.( 2005) Zmishana hnyl kartopli.[Mixed potato rot]. Karantyn i zakhyst roslyn. № 2. P. 24. [in Ukrainian]

Wollenweber, H.W.; Reinking, O.A. (1935) Die Fusarien, ihre Beschreibung, Schadwirkung und Bekämpfung. 1-355 [in German]

Kutsenko V. S. (2003). Kartoplya. Khvoroby i shkidnyky. [Potatoes. Diseases and pests] Kyiv. T. 2. P.240 [in Ukrainian]

Maryutin F.M., Pantyelyeyev, M.O. Bilyk F.M. (Maryutin F.M. Ed.) (2008). Fitopatolohiya: navch. posib. dlya pidhotov. fakhivtsiv napr. "Ahronomiya" u vyshch. navch. zakl. II-IV rivniv akredyt. M-va ahrar. polityky Ukrayiny [Phytopathology: textbook. tool. for preparation. professionals e.g. "Agronomy" in higher. teach. closed II-IV levels of accreditation. M-agrar. Politics of Ukraine]. Kharkiv: Espada. 552 p. [in Ukrainian]

Rudenko Yu.F., Polozhenets V.M. (2000) Sposoby polipshennia umov zberihannia bulb kartopli. Visn. Derzh. ahroekoloh. akad. Ukrainy: naukovo-teoretychnyi zbirnyk. 2000. №2. P. 298—303. [in Ukrainian]

Trybel S.O., Bondarchuk A.A. (2013) Metodolohiya otsinyuvannya sortozrazkiv kartopli na stiykist proty osnovnykh shkidnykiv i zbudnykiv khvorob. [Methodology for evaluation of potato varieties for resistance against major pests and pathogens]. Kyiv: Ahrarna nauka. 264 p. [in Ukrainian]

Bublyk L.I., Vasechko H.I., Vasylyev V.P. et al (Lisovyi M.P. Ed.) (1999). Dovidnyk iz zakhystu roslyn. [Handbook of Plant Protection]. Kyiv: Urozhay. 744 p. [in Ukrainian]

LeshchukN.V., Bashkirova N.V., Retman S.V., Serhiienko V.H., Kalenych F.S., Kyienko Z.B., Andriushchenko A.V. (ukladachi) (Tkachyk S.O. Ed.) (2016). Metodyka provedennia fitopatolohichnykh doslidzhen za shtuchnoho zarazhennia Roslyn [Methods of phytopathological investigations for artificial infection of plants]. Ukrainskyi instytut ekspertyzy sortiv roslyn. Vin­nytsia: Korzun D. Yu., 74 c. 76 p. [in Ukrainian]

Kyray Z., Klement Z., Shoymoshy F., Veresh Y. (1974) Metody fytopatolohyy. [Methods of phytopathology]. Moskva: Kolos. - 344 s. [in Russian]

Published
2019-12-06
How to Cite
Bomok , S. (2019). The resistance of potato varieties to dry fusarium rot in vitro. Quarantine and Plant Protection, (11-12), 25-28. https://doi.org/10.36495/2312-0614.2019.11-12.25-28
Section
PLANT DISEASES