Functional features of trophic use of carbon sources by pathogenic champignon bacteria (Agaricus bisporus)
Goal. Purpose. The study of the functional features of pathogenic bacteria, which are isolated from Agaricus bisporus.
Methods. The subject of the study is 16 isolates of pathogenic bacteria, which are isolated from Agaricus bisporus. They manifested as primary infections at the stage of active growth of the fungus. They are typically only for mushrooms. We used biotechnological methods. We isolated cultures of microorganisms to identify, sowing was carried out for splitting carbon sources on synthetic nutrient media with the adding of appropriate sugars. Using biochemical methods, trophic features of pathogenic bacteria were determined in order to develop biotechnologies for controlling their spread. The oxidase activity of bacteria was determined by the method of Kovach on a membrane filter previously moistened with NN-dimethil-p-pheniline diamine sulfate. Catalase activity was determined by adding to the culture drop a 10% solution of hydrogen peroxide. We used an innovative and universal method for determining the sources of carbon nutrition of microorganisms — КВ009 TM HiCarbo Kit.
Results. We have identified the sources of carbon nutrition of pathogenic bacteria using a test system. Isolate 9.4 utilizes sugars such as xylose, dextrose, galactose, melibiose L-arabinose, mannose, ONPG (ortho-nitrophenyl-β-galactopyranose), esculin, citrate, malonate. Isolate 6.2 when tested showed a positive result for xylose, dextrose. Isolate 6.1 uses such sugars: dextrose, trehalose, melibiose, mannose. Isolate 9.5 is indicated by the presence of ONPG, esculin, citrate and malonate and the absence of catalase. Isolates 11.1 and 9.5 have a positive oxidase reaction. Isolates 6.2 and 13.2 had a weak reaction. The reaction to the breakdown of carbohydrates showed that all isolates had an oxidative type of metabolism.
Conclusions. The biochemical properties of the reaction to the breakdown of carbohydrates in a synthetic nutrient media, the oxidase activity of bacteria by the Kovac method, the catalase activity and carbon nutrition sources of pathogenic bacteria isolated from the fruit bodies of Agaricus bisporus were studied.
Kalac P. A. (2013). Review of chemical composition and nutritional value of wild-growing and cultivated mushrooms. Journal of the Science of Food and Agriculture;93(2): P.209—218. doi: 10.1002/jsfa.5960 [in English].
Ivanova T.V. (2018). Biotechnologiya hrybiv. [Biotechnology of mushrooms]. V.2. Komprynt. 165 p. [in Ukrainian].
Sanguinetti M. (2015) Edible Mushrooms: Improving Human Health and Promoting Quality Life. International Journal of Microbiology. 2015: ID 376387, 14 p. doi: 10.1155/2015/376387. [in English].
Pereima, I. V., Ivanova, T. V. (2017). Stimulation of growth of species of the fungus of the genus Pleurotus (Fr.) P. Kumm. at a glucose nutrition. Biotechnologia Acta., 10(6), P. 45—52. doi: org/10. 15407 / biotech10.06.045. [in English].
Patyka V.P., Pasichnyk L.A., Dankevych L.A. (2014) Diagnostyka phytopatogennyh bakterii: Metodychni rekomendatsii. [Diagnostic of phytopathogenic bacteria: Guidelines]. Kyiv. 57 p. [in Ukrainian].
Ivanova, T.V. et all. (2015). New approaches extraction of viral RNA from edible mushrooms. Scientific Journal «ScienceRise».1 (15), P. 44—46. [in English].
Radchenko О. S. (2012). Fisiologo-biokhimichni vlastyvosti mikroorganizmiv ta metody yih vyznachennya: Navchalniy posibnyk. [Physiological and biochemical properties of microorganisms and methods their determination: Tutorial]. Kyiv: Agrar media group. 211 p. [in Ukrainian].
Patyka V.P., Pasichnyk L.A., Gvozdyak R.І., Petrychenko V.F., Korniychuk О.V., Kalinichenko A.V., Butsenko L.M., Zhytkevych N.V. (2017). Phytopatogenni bakterii. Metody doslidzhen. [Phytopathogenic bacteria. Research methods]. Vindruk. 432 p. [in Ukrainian].
Gadzalo Ya. M., Patyka M.V., Zaryshnyak A.S. (2015). Agrobiologiya ryzosphery roslyn. [Agrobiology of the rhizosfere of plants]. Kyiv: Agrarna nauka.368 p. [in Ukrainian].
Patyka M.V., Kolodyazhnii A.Yu., Ibatullin I.I. (2016). Otsinka metagenomu ta vyyavlennya funktsionalno znachuschyh polimorfizmiv prokariotiv gruntu metodom pirosektsionuvannya. [Metagenome evaluation and detection of functionally significant polymorphisms of soil prokaryotes by pyrosequencing]. Microbiologichnii journal, 78 (2). P.43—51. [in Ukrainian].
Patyka M.V., Patyka V.P., Patyka T.I., (2009). Filohenetychni vzayemozv'yazky mizh serolohichnymy variantamy Bacillus thuringiensis. [Phylogenetic relationships between serological variants of Bacillus thuringiensis]. Biopolimery ta klityny. 25 (3):С. 240—244. [in Ukrainian].
Orlova O.V., Vorobyova N.I., Svyrydova O.V., Andronov E.YE., Kolodyazhnyy A.YU., Moskalevsʹka YU.P., Patyka M.V. (2015). Sklad ta Funktsionuvannya mikrobnikh uhrupovanʹ pry rozkladanni solomy zlakiv u dernovo-pidzolystomu hrunti.[The composition and functioning of microbial communities in the decomposition of cereal straw in sod-podzolic soil]. Silʹsʹkohospodarsʹka biolohiya. 50 (3). P. 305—314. [in Ukrainian].
Patyka M.V., Kruhlov YU.V., Berdnykov A.M., Patyka V.F.(2008). Rolʹ Linum usitatissimum L. u formuvanni mikrobnykh uhrupovanʹ pidzolystykh gruntiv. Mikrobiolohichnyy zhurnal.. 70 (1). P 59—70. [in Ukrainian].
This work is licensed under a Creative Commons Attribution 4.0 International License.